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RT-qPCR analysis was performed on a QuantStudio 3 Real-Time PCR Detection System using ChamQ Universal SYBR qPCR Master Mix (Vazyme, Q711-02) with specific primers

Ipamorelin is typically administered via subcutaneous injection

GHK-Cu is a naturally occurring peptide found in human plasma that binds copper and plays a role in tissue repair and cellular support

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it is often diagnosed early by asymptomatic elevations in liver serum markers [alanine transaminase (ALT) and aspartate transaminase (AST)]