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4 and 5, Extended Data Fig

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The fact that adding cagrilintide produced meaningfully greater weight loss over just 32 weeks confirmed the additive effect of the dual mechanism

Western blot Equal amounts (50 g) of RINm5F, INS-1, and MIN6 cell lysate were analyzed by Western blotting with rabbit anti-AMPK monoclonal antibody#ab32047 (Epitomics Technology, Berlingame, CA), rabbit anti-phospho-AMPK(Thr172) monoclonal antibody #2531 (Cell Signaling Technology, Beverly, MA), rabbit anti-PARP polyclonal antibody#9542S, anti-cleaved caspase-3, phospho-CREB/CREB, PDX1, phospho Akt/Akt and GAPDH (Cell Signaling Technology, Beverly, MA), respectively

Tirzepatide pricing Compounded tirzepatide starts around $199 per month and can reach $499 per month at higher doses

The FDA must still complete a formal rulemaking process before licensed pharmacies can compound any of the six peptides under 503A