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As our cells create energy, they release what is known as reactive oxygen species, a toxin, which triggers oxidative stress

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Pregnant women require 1.5 g daily (the same as non-pregnant adults), whilst breastfeeding mothers need 2.0 g to support both their own requirements and those of their infant through breast milk

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Briefly, the frozen cortical samples were homogenized in SDT-lysis buffer (100 mM TrisHCl (pH 7.6) containing 0.1 M DTT) using an ultrasonic homogenizer, and the homogenates were heated at 95 C for 3 min