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After five washing steps with PBS-Tween, the secondary antibody (Alexa Fluor 555 anti-mouse, Life Technologies, 1:200) was added in 2% BSA/PBS-Tween together with Hoechst 33342 (1 g/ml) for 2 h
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Treatment duration varies by indication, with acute injuries typically requiring 2-6 weeks of therapy
While some people may notice improved energy or mental clarity within a week or two, the most significant benefits for liver function usually occur over several months of consistent use
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