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It is likely that these differences in the two methods arise from differences in sensitivity, given that our first mass spec experiment failed to identify any proteins when conducted using the same amount of input material as used for the redox arrays

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Proteins in total homogenates (2040 g) or nuclear extracts (40 g) were resolved by 10% SDS-PAGE and immunoblotted using following primary antibodies: chicken polyclonal antibodies against mouse GCLM 7 and mouse ALDH1A1 60

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Verbeke, R., Lentacker, I., De Smedt, S