An ACQUITY UPLC system (Waters, USA), equipped with a C18 column (AccQ Tag Ultra Column, 100 2.1 mm, 1.7 mm, Waters, USA) and a photo diode array (PDA) detector ACQUITY PDA 2996 was used for the chromatographic determination of reduced glutathione
Although most studies have focused on bacterial changes such as increased Streptococcus and Veillonella and decreased Faecalibacterium 63,64,65,66 , fungal involvement is supported by clinical observations of severe fungal infections in PSC patients 67,68 and serological evidence such as elevated ASCA in PSC populations 69
GSH-deficient cells showed increased Dnmt activity and decreased Tet hydroxylase activity (Fig
Its made up of the amino acids glutamine, glycine and cysteine
A.BornhorstJ.KuehneltD.SchwerdtleT
In contrast, cellular energy status sensing AMPK and deacetylase sirtuin 1 (SIRT1) are positive regulators of autophagy (Revuelta and Matheu, 2017)