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Males only have one copy of the G6PD gene and therefore are more likely to be affected by G6PD deficiency than females (who have two X chromosomes)

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After the membranes were incubated in blocking buffer at room temperature for 2 h, they were washed 3 times in tris-buffered saline and tween 20 (TBST) solution, 5 min each, and then incubated with primary antibodies overnight at 4 C

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