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B.PieckenstainF
The culture broth was centrifuged, after which the cell free extract was subjected for extraction of crude metabolites
Impaired prohormone processing: a grand unified theory for features of Prader-Willi syndrome
Cells were stimulated with increasing concentrations of ligand, 100 M forskolin or vehicle, and incubated for 30 min at 37C in 5% CO 2
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however, the overall weight loss results were superior to those in the placebo group
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