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After 24, 48 or 72 h exposure to experimental conditions, the conditioned culture medium (supernatant) was collected, and adherent cells were detached with 0.05% trypsin-EDTA
Importantly, the ability of the model to recover barrier integrity after transient permeability, is essential to provide a prototype for studying gut maturation overtime, particularly when assessing dietary (bioactive) components for their ability to accelerate this transition
Evid Based Mental Health 2012;15:11
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That said, signs or symptoms that you may be low in vitamin B12 include: fatigue pale skin headaches symptoms of depression stomach issues difficulty focusing or concentrating pain or inflammation in the mouth or on the tongue burning or pins and needles feeling in your hands or feet muscle cramps vision changes Learn more: Signs and symptoms of B12 deficiency
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