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The resulting supernatant was analyzed using high-performance liquid chromatography (HPLC, VWR Hitachi, Radnor, Pennsylvania, USA) coupled with mass spectrometry (3200 Q Trap, AB Sciex, Framingham, Massachusetts, USA) to quantify the target compounds

The pigmentation color may be tan, brown, blue, or black, but when multiple lesions are present, the color is fairly consistent among all of the lesions

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Activation of arcuate nucleus glucagon-like peptide-1 receptor-expressing neurons suppresses food intake