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According to the instructions of the Annexin V-FITC Apoptosis Detection Kit (Beyotime, C1062), 5 L of Annexin V-FITC reagent was added and gently mixed, followed by the addition of 10 L of propidium iodide staining solution, which was also gently mixed

N Engl J Med 2008;358:2545-2559

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