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The most effective time was 7 days of IF

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On the next day, after the membrane was washed with TBST for five times, HRP-conjugated secondary antibody (Thermo Scientific) was applied for 1.5 h at room temperature

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Developing smart delivery platforms for efficient glutamine metabolism disruption can minimize toxicity and improve therapeutic effects (Zhao et al., 2022)

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This finding has practical implications

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Many studies suggest that small, polar molecules may be able to bypass the bloodbrain barrier with nasal delivery, as the interface between the nasal cavity and brain is considered a potential point of vulnerability in the bloodbrain barrier

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