The most effective time was 7 days of IF
On the next day, after the membrane was washed with TBST for five times, HRP-conjugated secondary antibody (Thermo Scientific) was applied for 1.5 h at room temperature
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Developing smart delivery platforms for efficient glutamine metabolism disruption can minimize toxicity and improve therapeutic effects (Zhao et al., 2022)
This finding has practical implications
Many studies suggest that small, polar molecules may be able to bypass the bloodbrain barrier with nasal delivery, as the interface between the nasal cavity and brain is considered a potential point of vulnerability in the bloodbrain barrier