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FEMS Microbiol Lett 297:217224 Ishii J, Izawa K, Matsumura S, Wakamura K, Tanino T, Tanaka T, Ogino C, Fukuda H, Kondo A (2009) A simple and immediate method for simultaneously evaluating expression level and plasmid maintenance in yeast
The pain intensity ratings were assessed with a VAS for weekly average pain (VASavr) and maximum pain during the last week (VASmax)
Both are well-studied individually in preclinical models
Fluorescence intensity was measured at 530590 nm using a CLARIOstar microplate reader (CLARIOstar, BMG LABTECH, Germany)
Nat Rev Endocrinol 2021;17:757-66