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Jejunal tissue was isolated, first cleaned with PBS, then immediately flushed and stored in cold biopsy preservation buffer (BIOPS) (pH 7.1 (5.77 mM Na 2+ ATP, 10 mM Ca-EGTA buffer, 0.1 M free calcium, 6.56 mM MgCl 2 6H 2 O, 20 mM taurine, 20 mM imidazole, 60 mM K-lactobionate, 15 mM phosphocreatine, 0.5 mM DTT, 50 mM MES)

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The study populations with T2DM The analyses on the associations of semaglutide with both incident and recurrent AUD among patients with T2DM had a starting time of 12/2017 when semaglutide was approved in the US to treat T2DM as Ozempic and an ending date of 5/2021 to allow us to separately examine the associations of semaglutide on AUD as Ozempic from those as Wegovy in the study population with obesity