firemunki December 13, 2023, 4:23pm 21 Yeah, think Ill stick with my middle age tummy for now then
SNAP/FLAG-hGLP-1R plasma membrane recycling INS-1 832/3 SNAP/FLAG-hGLP-1R WT or V229A cells were seeded onto poly-L-lysine-coated black, clear bottom 96-well plates in full media, stimulated with vehicle or 100 nM exendin-4 for 1 hour at 37C to enable receptor internalisation, washed with PBS and incubated in 100 nM tetramethylrhodamine (TMR)-tagged exendin-4 (Ex-4-TMR) in full media for 1 hour or 3 hours at 37C to label any receptors that recycle back to the plasma membrane after agonist-mediated internalisation
Lost muscle lowers your resting metabolism, which makes weight maintenance harder and regain easier
It is a naturally occurring tripeptide, meaning it consists of just three amino acids bound to a copper ion
GLP-1 medications can be a useful part of that journey for some women, and lifestyle changes can help carry those benefits into a healthy pregnancy and beyond
Zhang Y, Parajuli KR, Fava GE, et al