If the patient is suspected to be sensitive to cobalt or other components of cobalamin, an intradermal test dose is recommended.[12][15] Several laboratory tests should be performed prior to treatment with cyanocobalamin, including serum vitamin B12, folate, iron, hematocrit, and reticulocyte count
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Plasma levels decline significantly with age from approximately 200 ng/ml at age 20 to roughly 80 ng/ml by age 60
Curr Pharm Des (2013) 19:495363
Jones DP, Coates RJ, Flagg EW, et al
TM3 cells were seeded in 96-well plates at a density of 510^3 cells/well