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GSH or Ni-NTA bead washing was performed four times with cold PBS or washing buffer (20 mM Tris-Cl (pH 8.0), 300 mM NaCl, 20 mM imidazole)

doi: 10.1016/j.mam.2004.02.005 6 GalarisDPantopoulosK

Toxicol Appl Pharmacol 285(2):136148 McKinsey TA, Foo R, Anene-Nzelu CG, Travers JG, Vagnozzi RJ, Weber N, Thum T (2022) Emerging epigenetic therapies of cardiac fibrosis and remodelling in heart failure: from basic mechanisms to early clinical development

Ensuring prompt medical intervention can prevent permanent damage and stabilize the individuals condition

Overall, side effects are rare, and taurine therapy is considered very safe within recommended dosing ranges

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