For p H titration assays 34 , different buffers with a p H from 2.0 to 10.0 were prepared at a normality of 0.1
As expected, hepsin / mice displayed heightened oxidative stress after APAP exposure compared to wild-type mice, indicated by a significant decrease in GSH and an increase in nitrotyrosine levels, leading to more severe liver damage and mortality
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However, when there is an excess of these unstable molecules, their high reactivity can cause damaging chain reactions
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