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However, the sample available at the 6-year follow-up was not entirely comparable to the one selected for the main analyses, as it included subjects with a substantially better health profile (indicative of survival bias) compared to those lost to follow-up
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[1] [2] Common GLP-1 medications include semaglutide (Ozempic, Wegovy), dulaglutide (Trulicity), liraglutide (Victoza, Saxenda), while tirzepatide (Mounjaro, Zepbound) is a dual GIP/GLP-1 receptor agonist
The cells were lysed using sonication in BugBuster Protein Extraction Reagent (Merck Millipore, Darmstadt, Germany), and GST, GST-GIP, and GST-GLP-1 proteins were purified as previously described 10