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PMID 11076863
Investigating an underlying condition that has not yet been ruled out
Warning Signs of Improper Dosing TTaking too little B12 may leave deficiency-related nerve symptoms untreated, while improper use can also lead to unwanted effects or missed diagnoses
The concentrations of protein extracts were determined by using the BCA Protein Assay Kit (Thermo Fisher Cat #23225), then protein samples were mixed with 3 loading buffer and loaded in equal amounts into the wells of 412% Bis-Tris protein gels (Bio-Rad) to transfer the proteins to PVDF membranes (Millipore)
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Genazzani AD, Despini G, Czyzyk A, Podfigurna A, Simoncini T, Meczekalski B
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