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The Journal of Biological Chemistry, 289(28), 1950819518
Hyaluronic Acid
In recent years, GHK-Cu has evolved from a dermatological bioactive ingredient into a representative model system linking coordination chemistry, peptide bioactivity, and biomanufacturing engineering
At this stage of purification GST-X fusion should be clean and depending on the nature of furhter experiments that we want to perform, we can either elute the fusion with glutathione (excess of the glutathione will compete and displace GST-X protein from the beads) or cleave the GST tag and release protein X (see cartoon below)
J Neuropathol Exp Neurol 69:155167 Sultana R, Piroddi M, Galli F, Butterfield DA (2008) Protein levels and activity of some antioxidant enzymes in hippocampus of subjects with amnestic mild cognitive impairment
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