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The homogenate were further diluted with another three fold volumes of PBS without NEM, and mixed with the same volume of 10% (v/v) TCA
The consequences are molecular, irreversible, and invisible
Aagaard et al., Growth hormone and growth hormone secretagogue effects on nitrogen balance and urea synthesis in steroid treated rats, Growth Horm
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doi: 10.1038/s41598-021-86489-4 80 FerreiraA
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