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Proteins were eluted by adding an elution buffer [50 mM tris (pH 7.5), 500 mM NaCl, 2 mM 2-mercaptoethanol, and 500 mM imidazole]
Additional information Edited by A Finazzi Agr Rights and permissions About this article Cite this article Laborde, E
[26] See also [edit] References [edit] "Large conformational changes in the catalytic cycle of glutathione synthase"
Sonveaux, P
thymidylate/methionine biosynthesis)
Hepatol Res 34:5763
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