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Global protein abundance profiling by mass spectrometry Sample preparation (A) Flash frozen liver sections (~25 mg) from WT or Gclc / mice (3 biological replicates each per TMT sample) were thawed on ice and resuspended in DPBS, supplemented with protease (cOmplete, EDTA-free protease inhibitor cocktail, Roche, #11873580001) and phosphatase (PhosSTOP, Roche, #4906845001) inhibitor tablets

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"If someone is losing weight quickly while skipping meals, eating very little protein and not strength training, they may be at higher risk for losing muscle," says registered dietitian Serena Pratt, MS, RDN

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