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For quantitative PCR (qPCR), total RNA was reverse transcribed into complementary DNA using a Maxima H Minus First Strand cDNA Synthesis Kit (Thermo Scientific, K1682) following the manufacturers instruction

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The primary mechanisms we see cited in the literature include: VEGF upregulation: BPC-157 increases vascular endothelial growth factor, which drives new capillary formation in injured tissue

Independent clinical literature notes that BPC-157 injection dosing varies by indication and provider protocol

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