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a pool of three individual siRNAs: 5-GGAAAUCCAGCAACAUGAA-3, 5-GACGUAUGGUGCUUGGUAU-3, 5-GCAUCAGCAAGACUCAGAA-3), Nesprin-2 (Syne-2

After washing, 50 L/well of HRP-conjugated detection antibody (anti-IgM-HRP Sigma A8786) was added for 1 h at RT

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