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Here is what researchers need to know about GHK-Cu

The concentrations of reduced (GSH) and oxidized (GSSG) forms of glutathione were measured using an HPLC system (Thermo Fisher Ultimate 3000) equipped with a YMC-Pack ODS-A column (5 m, 120 , 4.6 150 mm

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Add 20 l of Substrate working solution, and incubate the plate at 37C for 20-40 min

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