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We utilized Superscript III reverse transcriptase enzyme (Invitrogen) with random hexamer primers and 3 g of RNA from each sample to produce cDNA according to the manufacturers protocol

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However, specific injuries such as traumatic brain injury, stroke, brain tumor, or aging are believed to compromise the efficiency of BBB protection, thereby allowing MSCs access to cross the BBB and deliver their protective effects

10.1007/s10787-017-0323-6 76 MihovilI.RadicB.BrcicL.BrcicI.VukojaI.IlicS.et al (2009)