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Mushrooms treated with 1% glycerol exhibited the lowest water activity (aw) compared to those treated with 0.1% potassium metabisulphite, 0.2% potassium metabisulphite, 1% CaCl 2 , 2% CaCl 2 , and 0.5% glycerol

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The p53 pathway genes, namely, BAX, p21, MDM2, and TP5313, which are involved in DNA repair, apoptosis, and cell cycles were also up-regulated with EGCG treatment (Zhang et al., 2010a)

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The power to detect differences between the study groups is larger than what is projected here as we will measure most of the outcomes more than once and also use these repeated measurements in the same analyses, adjusting for interdependence within a child using mixed effects models, generalised estimating equations or other appropriate methods