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James, Free Radical Biology and Medicine, 2008 Limites de la prise de NAC pour la synthse de glutathion : N-acetylcysteine: clinical applications and mechanisms of action , H

10.1124/jpet.104.065607 23 DiasM

However, TRIM28 peaks only partially and inconsistently overlapped with regions of increased ATAC-seq signal or AP2 binding sites, suggesting that additional mechanisms beyond the currently proposed TRIM37TRIM28AP2 model may contribute to the regulation of somatic gene repression in PGCs

Western blot analysis Homogenized liver and spleen tissues, and splenocytes from spleen of WT mice and Jurkat T cells lysed by protein extraction solution (PRO-PREP, iNtRON Biotechnology) containing protease inhibitor cocktail (Calbiochem, Darmstadt, Germany) and phosphatase inhibitor cocktail (Roche, Basel, Switzerland)

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Biological replicates ( n = 4 for each group) were multiplexed using TotalSeq-A Cell Hashing Antibodies