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NMR relaxation experiments for the recombinant L -version of the FOXO4-DRI sequence were recorded using 13 C, 15 N-labeled samples at 300 M in a buffer containing 50 mM NaH 2 PO 4 /Na 2 HPO 4 , pH 6.5, 0.04 % NaN 3 , 2.5 mM DTT and 10 % D 2 O in the absence and in presence of 400 M synthetic D -p53 TAD2 peptide using the same NMR parameters
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Assays were performed by at least three technicians using two lots of components
