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freezing causes ice crystal formation that irreversibly shears peptide chains

Blocked and permeabilized sections were then incubated overnight at room temperature with rabbit anti-GABA A R-1 (1:500, EMD Millipore, Billerica, MA, catalog# 06868)

Int J Neurosci Khvatova EM et al

To capitalize on the full therapeutic benefits of adjunctive treatment by including Se or prebiotic therapy in the medical management of GD, more well-designed, appropriately powered clinical trials are required

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