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Nat Cell Biol 16(11):10691079 Du X, She E, Gelbart T, Truksa J, Lee P, Xia Y, Khovananth K et al (2008) The serine protease TMPRSS6 is required to sense iron deficiency
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After transduction with the relevant guide-containing plentiCRISPRv2, and selection for cells resistant to puromycin (Invivogen, 2 g/mL), single cell ACSL1 -deficient clones were isolated by plating cells in 96-well plates at a concentration which 1 cell per 3 wells would be expected
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