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Once complexed with Cu(II), the peptide backbone rearranges into a specific square-planar coordination around the copper ion, with donor atoms from the glycine amino terminus, the deprotonated amide nitrogen, the histidine imidazole, and a fourth position (carboxylate or water depending on pH)

[2] [16] Dietary deficiency without neurological features: Initial loading doses followed by maintenance therapy, with consideration of oral supplementation in appropriate cases

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Second, shaking creates foam

Van Dam, R.S

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