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2.15.2 Neural function defect score (NSS) Twenty-four hours post-infarction, neurological deficits were scored using the Masao Shimizu Sasamata method ( 2.16 Immunohistochemical analysis Mice were humanely sacrificed by deep anesthesia using an overdose of pentobarbital, followed by transcardial perfusion with phosphate-buffered saline (PBS) to clear the blood

The same test can mean something different depending on whether it's answering "what's wrong right now," "is something starting to go wrong," or "is this working the way it should?" 1

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Handling and Storage Recommendations Keep lyophilized vials sealed at 20C or colder, dry, and protected from light

Preclinical safety evaluations revealed no toxicity in acute studies with SD rats and beagles following intravenous/intramuscular administration [10]

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