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Briefly, after 24 h transfection with various constructs, HEK 293T cells were digested by 0.2% (w/v) EDTA and washed once with Dulbeccos phosphate buffered saline (DPBS)
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Weir GC, Bonner-Weir S
Collectively, the binding mode comparison of the three peptides bound by the same receptor demonstrates common structural features in ligand recognition and distinct conformational adaptability of GIPR, GLP-1R and GCGR in response to different agonist stimulation
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