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Per HDR reaction, 3.75 l of 1 M gRNA, 0.125 l of 5 g/l TrueCut Cas9 v2 (A36498

Immunofluorescence analysis on direct ECFC-MSC two-dimensional co-culture For the ECFC-MSC co-culture, cells were mixed at a ratio of ECFC: MSC = 1:5 and grown on coverslips in complete EGM-2 + 10% FBS and DMEM + 20% FBS at a ratio of 1:5

Cycle length 8 to 12 weeks on, 4 to 6 weeks off in common research community protocols

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Accurate dosing is essential

[2] These doses are much higher and are given under close medical supervision