Slices were anchored in a glass-bottom chamber using a harp (Warner instruments) and imaged using a 40 water immersion objective
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Studies also indicate that the activation patterns of particular brain regions in patients with depression, especially during emotion and reward processing, are strongly associated with gene expression patterns
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Of the other sites we propose, some are more likely than others, but those in TXNRD1 and SELENOF are particularly convincing, and all of the predicted sites map to surface accessible regions of the targeted proteins (Figure S3)
Bru-Seq data were further analysed using GSEA for exploring the mechanism of cellular action of GSTO1 and its inhibitor