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Results Generating and identifying recombinant pDNA/polybia-MP1 The construction of the recombinant pDNA/polybia-MP1 plasmid involved incorporating the polybia-MP1 gene into the pcDNA3.1(+) expression plasmid (Fig

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FIGURE 4 To characterize the potential biological function of sFNDC5, we prepared its recombinant protein (r-sFNDC5) using a Pichia pastoris expression system and the protein was purified from the clear medium using a Ni-NTA chromatography column (Zhang et al., 2014)