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45% fat, 35% carbohydrates) for 21 days, followed by a single low intraperitoneal dose of streptozotocin (STZ, 10 mg/kg body weight) in 0.1 M sodium citrate buffer (pH 4.5) 33

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Given that the effectiveness of NAC for treating APAP-induced liver injury is confined to modest improvements within a brief period after onset and considering that high doses of NAC can potentially negatively impact hepatocyte metabolism and liver regeneration (Jaeschke et al

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