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The procedure is fast, and any soreness typically fades within a few hours

The samples were incubated for 1 h at RT, washed three times with TBST, reacted with Supersignal West Pico solution (Pierce) for 5 min, and exposed to X-ray film (Agfa)

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By bypassing the cell surface processing of GSAO, PENAO accumulated 85 times faster in cells (Dilda et al., 2009)