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Mice were exposed to CS for 3 days to achieve an adaptive GSH level of roughly 250 M and they were sacrificed immediately after the third day of CS exposure or after 16 hours of rest (Figure 6)
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The serum was aliquoted and promptly frozen at -80 C
These observations highlight the need to better understand barrier dysfunction in PD and its potential as a therapeutic target [177,178,179,180]
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