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Concentration honesty: Clinical studies used 0.01 to 1% topical

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Sucrose Gradient Sedimentation of the Cytosolic TuRC HEK293T cells, grown to confluence, were washed with HBS (50 mM HEPES, pH 7.4, and 150 mM NaCl) and were then lysed at 4C in HBS supplemented with 1 mM DTT, 1 mM EGTA, 1 mM MgCl 2 , 0.25 mM GTP, 0.5% Triton X-100, and the Protease Inhibitor Cocktail

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