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Flow cytometry and sorting For surface staining cells were incubated at 48 C, in the dark with fluorochrome-conjugated CD19, CD23, CD21-CD35 or CD35, IgD, IgM, CD1d, CD24, CD93, CD95, and GL-7 diluted 1:200 (refer to Supplementary Data 4 for details)

Verify checklist (bring to your next visit): Do I have lab evidence of a deficiency

All references are limited to laboratory and experimental contexts

Once hot flashes have improved, sleep is better, and hormone levels are confirmed in the therapeutic range, begin tirzepatide at the starting dose of 2.5 mg weekly

10.1016/j.molmet.2019.09.010 55 MurphyR.ColcloughK.PollinT

159 Anakinra, a recombinant IL-1 receptor antagonist (IL-1Ra), competitively inhibits the binding of IL-1 and IL-1 to IL-1 receptors (IL-1Rs), thereby suppressing IL-1-mediated inflammatory signaling