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Both of these injection sites contain major arteries (the jugular and femoral, respectively), and it can be deadly to pierce them by mistake
It can push stem or progenitor cells towards repair phenotypes and it advances regeneration-specific cell cycle genes
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After incubation, cells were stained with propidium iodide (PI, # 40710ES03, Yeasen) solution (40 g/mL in PBS) for 15 min at room temperature and the cell cycle was analyzed using Flowsight flow cytometer (Merck Millipore, Darmstadt, Germany)
Allow both the vial and bacteriostatic water to reach room temperature
Godiksen, A., Vennestrom, P., Rasmussen, S
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