EP thanks the NRF of South Africa (grant number 142142) and SA MRC (self-initiated research (SIR) grant)
The role of incretins on insulin function and glucose homeostasis
Immunoblotting Proteins were extracted from cultured SCD erythroblasts or spleen Ter119+ erythroblasts with modified RIPA buffer (150 mM NaCl, 50 mM Tris, pH 7.5, 1% NP40, 0.25% Na-deoxycholate) plus protease inhibitor cocktail (Sigma) and stored at 80 C up to processing
Amy Rothberg, clinical professor of internal medicine in the Division of Metabolism, Endocrinology and Diabetes at the University of Michigan, says it also helps improve outcomes in people with type 2 diabetes and cardiorenal (heart and kidney) conditions, similar to semaglutide
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Plasmids For knockdown studies, gene-specific shRNAs were cloned into pLKO.1 vectors (puro/hygro