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In animal studies, typically involving rats and mice, doses range from 0.1 to 10 micrograms per kilogram of body weight, administered via intramuscular, subcutaneous, or oral routes

For comparison, sulfasalazine (50 mg/kg intraperitoneally, once daily) (moderately effective) or 6--methylprednisolone (1.0 mg/kg intraperitoneally, once daily) (aggravation) was given

However, certain drugs may affect how your body uses B12, making injections particularly beneficial

The molecule was first identified and studied by the Sikiric research group in Croatia starting in the early 1990s, and the bulk of the preclinical evidence base comes from more than three decades of rat and rodent studies showing consistent tissue-repair, anti-inflammatory, and gastroprotective effects