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Several amino acids had their concentrations increased following MTX treatment: glycine, alanine, phenylalanine, threonine, histidine, valine, arginine, leucine, isoleucine, tyrosine, and proline

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doi:10.1515/CCLM.2007.362

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Stick to standard reconstitution volumes that balance stability with practical measurement

This makes bacteriostatic water the standard choice for multi-dose vials, as it allows the solution to be stored and used for several weeks after the initial mixing