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The OD value is proportional to the concentration of GPX1

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To determine whether semaglutide-induced weight loss alters skeletal muscle mitochondrial content and oxidative capacity, we next assessed the abundance of mitochondrial OXPHOS complexes and citrate synthase activity

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BCTC, a highly potent TRPV1 antagonist that competitively interacts with CAP to bind to TRPV1 channels 40 , was used for this assay