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Using Sterile Water Instead of BAC Water Sterile water works but has no preservative

However, observational studies suggest that whilst acute toxicity remains exceptionally rare, sustained very high levels of B12particularly from supplements or injectionsmay be associated with certain health concerns, though these are correlations rather than proven causal relationships

B12 plays a vital role in converting food into usable energy by aiding the metabolism of carbohydrates, fats, and proteins

It depends entirely on the final concentration you want to achieve for your research protocol

doi:10.3390/nu2030299

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